CHARACTERIZATION OF NOVEL HIGH-QUALITY RICE GERMPLASM AND MOLECULAR MARKERS ASSOCIATED WITH AROMA AND AMYLOSE CONTENT

The success of the breeding strategies needs to exploit a new genetic diversity of various diverse sources. The diverse set (350) of improved varieties, landraces and promising lines, is collected by the Genebank of Cuu Long Delta Rice Research Institute (CLRRI). Our research provides for breeder the necessary characteristics of rice varieties such as days to mature, yield, yield components, aroma, and amylose content. Using the molecular marker-based PCR analysis of 350 diverse accessions, are linked to the specified traits of amylose AC and aroma. Two markers of Waxy gene consist of the (CT)n repeat (RM190) and A/C SNP of exon 6 are tightly associated with amylose content. The results of RM190 showed that 262 accessions with low AC content, 137 accessions high AC, 1 accession heterozygosity. SNP_exon6 is closely related to the intermediate amylose content (140 accessions). 276 rice varieties for BADH2 non-aromatic, 74 fragrant varieties (71 score 1, 3 strong aroma). Multivariate analysis was done by PCA and cluster analysis, found large diversity and variable in the gene pool. These results should have priority for the plant breeder to explore the genetic variation of the amylose content, aroma trait and the other traits.