CHARACTERIZATION OF WINGED BEAN BASED ON MOLECULAR MARKERS

Twenty winged bean genotypes received from All India Co-ordinated Research Network on Potential Crop, Department of Agricultural Botany, MPKV, Rahuri, MS, India were sown and used for assessment of molecular polymorphism, diversity analysis by 11 ISSR primers. All primers successfully amplified polymorphism regions, as among 124 bands position, 76 polymorphic regions (61.3%) were amplified. Accordingly, highest and lowest similarities among genotypes were measured as 98% and 72% respectively. The results based on UPGMA method also categorized all genotypes into four main groups. The genetic distance based on the ISSR markers indicated the close relationship and narrow genetic background between winged bean accessions.