GENETIC VARIATION OF WILD RICE IN VIETNAM

The study aimed at identifying rice gene pools carrying blast resistant genes via phenotyping and genotyping for wild rice in Vietnam. The experiments were carried out at CLRRI (Cuu Long Delta Rice Research Institute) laboratories, greenhouses and experimental fields, Thoi Lai, Can Tho, from 2011 October to 2015 October. Major research activities consisted of evaluating the genetic diversity of wild rice accessions. A total of 101 accessions of some wild rice species in the genebank of Cuu Long Rice Research Institute, Vietnam were used to clarify the diversity using SSR markers. Molecular diversity analysis using 38 polymorphic SSR markers revealed among the 101 accessions. The 101 Vietnam accessions generated 2 clusters at 0.76 similarity coefficient. Some with the same variety names were grouped into different clusters. Genotyping blast resistance among wild rice via molecular markers as SSRs (simple sequence repeats) showed the results of a total of 101 wild rice accessions were classified into two cluster groups A , and B based on the polymorphism data for 120 alleles of 38 SSR markers. Te Tep varieties including O. punctala-1 , O. latifolia-1, O. nivara-1 were categorized into group B. The other two groups A (98 accessions) as an LTH -type control was also categorized into group A. Phenotyping blast resistance among wild rice for blast isolates collected from the Mekong Delta. Against 13 standard differential blast isolates which were selected based on the reactions to monogenic lines for targeting 101 resistance genes as the differential accessions, these resistant of rice accessions were investigated. Based on the cluster analysis, these accessions were classified into three groups, A, B and C.