OVEREXPRESSION OF GMCHS7 IN SOYBEAN LEADS TO REDUCED PIGMENTATION IN HILUM OF TRANSGENIC SEEDS

GmCHS7 gen was cloned and transferred to DT22 soybean plants by agrobacterial vector. Transgenic plants were selected based on their resistance to ammonium glufosinate conferred by the bar gene which is co-expressed in the T-DNA. The presence of transgene in surviving plants were further confirmed by using PCR. Transgenic plants showed no morphological difference from DT22 variety except for hilum color which ranges from white to brown in the seeds of transgenic plants compared to black or imperfect black in that of DT22 plants. The results showed that overexpression of GmCHS7 gene lead to a decreased in pigmentation of the hilum in the seeds of soybean plants.